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Exoproteolytic activity determined by flow injection analysis: its potential importance for bacterial growth in coastal marine ponds ArchiMer
Delmas, Daniel; Legrand, Catherine; Bechemin, Christian; Collinot, Cecile.
The measurement of the fluorescent 4-methyl-7-coumarinylamine released from the hydrolysis of a non-fluorescent peptide model substrate by exoproteolytic enzymes has been adapted to flow injection analysis (FIA). FIA allows samples to be processed very quickly (less than 2 min. for triplicate determinations) with good sensitivity (< 0.1 muM) and reproducibility (relative standard deviation < 3 % at the 0.2 muM level). In a coastal marine pond, exoproteolytic activity was closely related to bacterioplankton biomass. The high activity measured in pond water (maximum velocity: V(M) almost-equal-to 1.46 to 2.54 muM.h-1) emphasizes the importance of dissolved protein hydrolysis for bacterial growth and for dissolved organic nitrogen cycling. The mean...
Tipo: Text Palavras-chave: Activité exoprotéolytique; Protéine dissoute; Analyse en flux continu; Bactérioplancton; Marais maritime; Flow injection analysis; Exoproteolytic activity; Dissolved protein; Bacterioplankton; Coastal marine pond.
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00196/30756/29163.pdf
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Les virus de l'estuaire de la Charente el du bassin de Marennes-Oleron. Dynamiques spatio-temporelles et interactions avec les communautés bactériennes ArchiMer
Auguet, Jean-christophe.
This study is divided in two parts; one aim is to evaluate the in situ spalio-temporal dynamics of viral communities in the Charante estuary in order to define the appropriate seasons to conduct in vitro viral enrichment experiments. These experiments, that constitute the second part of this study, were performed to assess the impact of freshwater viruses carried by the Charante River on the dynamics and composition of coastal bacterial communities. With abundance ranging from 1.4 x 107 to 208 x I07 VLPml-1. viruses are the most abundant component among plankton microorganisms in surface waters of the Charente estuary. Thre temporal dynamics of this component reflects the indirect influence of temperature through the control of bacterial abundance,...
Tipo: Text Palavras-chave: Richesse spécifique; Composition clonale; Enrichissement vital; Infection lyrique; Structure des communautés; MET; PPGE; Dynamique temporelle; Gradient de salinité; Estuaire; Bactérioplancton; Virioplancton; Species richness; Clonal composition; Viral enrichment; Lytic infection; Community structure; Diversity; TEM; PEGE; Temporal dynamic; Gradient; Salinity; Estuary; Bacterioplankton; Virioplankton.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/these-3071.pdf
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